![]() In my research, I developed a method called photoactivated localization microscopy (PALM), which enabled us to break that limit with the help of glowing (fluorescent) molecules. This meant challenging a limit that was long believed to be unbreakable. This is why I chose to work on one of the most difficult problems in the field of optical (light-using) microscopy-how to see objects that are smaller than the wavelength of visible light. ![]() When I started my scientific career, I did not want to do something small-I wanted to do something that would be really different and impactful. ![]() I was always drawn to heroic characters that invented something new that resulted in an amazing breakthrough. * I was young, I wanted to be an astronaut and I was a hard-core science fiction fan. Betzig joined the faculty of the University of California, Berkeley, where he is currently working on other microscopes to reveal the detailed workings of living cells. Betzig joined the Howard Hughes Medical Institute, where he continued to work on super high-resolution fluorescence microscopy. Betzig and his friend Harald Hess started developing a high-resolution optical imaging method called photoactivated localization microscopy (PALM), for which he won the Nobel Prize in chemistry in 2014. After exhausting that route of research, and as a result of advancements in the field of fluorescent molecules, Prof. He then went on to work on near field microscopy at Bell Labs for several years. Betzig studied physics at the California Institute of Technology and earned his doctorate in applied physics at Cornell University, focusing on near field microscopy as means for breaking Abbe’s limit of resolution in classical optical microscopy. Eric Betzig is a professor of physics and molecular and cell biology at the University of California, Berkeley, and a senior fellow at the Howard Hughes Medical Institute, Janelia Farm Research Campus, United States.
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